Gertsch Group

Institute of Biochemistry and Molecular Medicine, University of Bern, Switzerland

Services / Methods

Image Methods Section

SERVICES (cooperations) currently offered

  • Cytotoxicity screening (selected panel of cancer cell lines), determination of IC50 values 72 h (antiproliferative effect)
  • Detection of apoptosis
  • Cell cycle analysis
  • Different assays to determine interaction with microtubules / alpha, beta-tubulin in vitro and in cellular systems
  • Determination of interaction with cannabinoid CB1 and CB2 receptors (Ki values, competitive vs. non-competitive ligands)
  • Quantification of pg/ml endocannabinoids (anandamide, 2-AG, noladin ether) by LC-MS/MS and related lipids and steroids

Methods

  • Quantification of endocannabinoids in cell cultures and other matrices, such as brain and plasma (LC-MS/MS, GC-MS)
  • Validated assay to measure endocannabinoid transport (uptake) into cells (radioactivity, TLC, GC-MS)
  • Scintillation proximity to monitor lipid transport accross cell membranes
  • Different FACS techniques
  •  Activity-based protein profiling (ABPP)

SPECIAL OFFER: We sell dimethylisopropylsilyl imidazole at a very low price, 1/3 of the price TCM offers it to you.

 

Equipment Gertsch Lab

  • Cell culture facility
  • Western blotting facility
  • FACScan BD flow cytometer equiped with a solid state laser (Cytek
  • LC-MS/MS ABISciex 4000 with UPLC Shimadzu
  • Nikon microscope TS100 fluorescence and video camera NIS Elements D
  • TopCount 96-well scintillation plate reader
  • Microbeta multiwell and multichannel scintillation reader
  • Robotics (screening)
  • Aurora VIPR II Ion probe HTS system
  • HPLC diode array HP1090 and fluorescence detector
  • Rotavapors Büchi, UV/VIS TLC viewing system
  • Lyophilizator multiple ports
  • Camag Linomat 4 Sampler and TLC plotter
  • Typhoon FLA9500
  • GC/MSD-Systems Serie 6890N/5975C Agilent
  • 96-well FACS (FACScan equiped with a solid state laser (Cytek) with an AMS (automatic microsampling system) robot
powered by Doodlekit™ Free Website Maker